1. Produktua amaitu daview
The Labnet Enduro Gel XL Electrophoresis System is a comprehensive solution designed for horizontal gel electrophoresis. It includes all necessary components for casting and running gels, ensuring efficient and reliable laboratory procedures. This system is engineered for user convenience and safety, featuring a direct power supply connection, robust construction, and advanced safety mechanisms.
Figure 1: Labnet Enduro Gel XL Electrophoresis System, showing the clear gel tank connected to the power supply unit with a digital display.
2. Osagaiak barne
The E0160-230V Enduro Gel XL system comes complete with the following components:
- Gel Tank (Migration Tank)
- Elikatze Hornidura Digitala
- Gel Trays (various sizes: 12.5 x 12cm, 12.5 x 6cm, 6 x 6cm)
- Combs (multichannel)
- Casting Stand
3. Ezaugarri nagusiak
The Enduro Gel XL system incorporates several features designed for optimal performance and user safety:
- Diseinu Integratua: Direct connection of the power supply into the gel box eliminates the need for external power leads, simplifying setup and reducing clutter.
- Eraikuntza sendoa: The migration tank, gel trays, and multichannel combs are molded for leak-proof performance and extended service life.
- Flexible Power Control: The power supply can be set to either constant voltage or constant current, with automatic crossover between the two parameters for precise control.
- Garbitu pantaila digitala: Parameters are shown on a large digital display, ensuring easy visibility from a distance.
- Versatile Gel Options: Supports three different gel sizes: 12.5 x 12cm, 12.5 x 6cm, and 6 x 6cm. Capable of running one large gel (up to 112 samples), two small gels, or four micro gels simultaneously.
- Electrode Protection: Cassettes protect fragile electrodes from damage and allow for easy replacement.
- Segurtasun hobetua: A magnetic sensor ensures current flows to the electrodes only when the lid is securely in place. If the lid is removed during operation, current is immediately shut down.
- Beroa xahutzea: The lid of the gel box is vented to effectively dissipate heat generated during electrophoresis, maintaining stable operating conditions.
- Ikusizko jarraipena: A clear window over the gel platform allows for easy visualization of the gel while it is running.
4. Konfigurazioa eta Muntaketa
Follow these steps to set up your Enduro Gel XL Electrophoresis System:
- Despaketatzea: Kontu handiz atera osagai guztiak paketetik. Begiratu ea kalterik dagoen.
- Casting Gel:
- Place the desired gel tray into the casting stand.
- Insert the appropriate comb(s) into the designated slots on the gel tray.
- Pour the prepared agarose gel solution into the tray. Ensure no bubbles are trapped.
- Allow the gel to solidify completely.
- Transferring Gel: Once solidified, carefully remove the comb(s) and the gel tray with the cast gel from the casting stand.
- Placing Gel in Tank: Position the gel tray with the cast gel into the gel tank. Ensure it is seated correctly.
- Adding Buffer: Pour electrophoresis buffer into the gel tank until the gel is fully submerged and the buffer level reaches the recommended mark.
- Elikatze-iturria konektatzea: Align the gel tank with the power supply unit and gently press them together until they click into place. The direct connection ensures proper electrical contact.
- Estalkiaren kokapena: Securely place the vented lid onto the gel tank. The magnetic sensor will detect the lid's presence, enabling current flow.
5. Funtzionamendu-argibideak
Operating the Enduro Gel XL system involves setting the electrophoresis parameters and initiating the run:
- Piztu: Ensure the system is properly connected to a 230V power outlet. The digital display on the power supply will illuminate.
- Ezartzeko parametroak:
- Use the control buttons to set the desired voltage (up to 150V) or current (up to 400mA).
- Select the operating mode (constant voltage or constant current). The system will automatically crossover if the other parameter limit is reached.
- Set the run time if desired.
- Verify all parameters on the digital display.
- S kargatzenamples: Carefully load your DNA/RNA samples into the wells of the gel using a micropipette.
- Start Run: Press the 'Start' button on the power supply to initiate the electrophoresis run. The display will show the active parameters and remaining time.
- Jarraipena: Observe the migration of samples through the clear window. The vented lid helps dissipate heat.
- Stopping Run: The system will automatically stop when the set time is complete. Alternatively, press the 'Stop' button to manually end the run.
- Deskonektatzea: Press the eject button to separate the gel box from the power supply.
- Gel Retrieval: Carefully remove the gel tray from the tank for further processing (e.g., staining, imaging).
6. Mantentzea eta Zainketa
Proper maintenance ensures the longevity and optimal performance of your Enduro Gel XL system:
- Garbiketa: After each use, thoroughly rinse the gel tank, gel trays, and combs with deionized water. For stubborn residues, use a mild laboratory detergent and a soft brush. Avoid abrasive cleaners.
- Elektrodoen zaintza: Periodically inspect the electrodes within their cassettes for any signs of wear or corrosion. Replace electrode cassettes if damage is observed.
- Elikatze-iturria: Wipe the exterior of the power supply unit with a damp cloth. Do not immerse the power supply in water or any cleaning solutions.
- Biltegiratzea: Store all components in a clean, dry place when not in use. Protect from direct sunlight and extreme temperatures.
- Ikuskapena: Regularly inspect all components for cracks, leaks, or other damage. Do not use damaged components.
7. Arazoak
Atal honek aurki ditzakezun arazo ohikoenak jorratzen ditu:
| Arazoa | Kausa posiblea | Irtenbidea |
|---|---|---|
| Sistema ez da pizten. | No power to outlet; power supply not connected properly; internal fuse. | Check power cord and outlet. Ensure power supply is securely connected to the gel tank. If issue persists, contact technical support. |
| No current flow during run. | Lid not properly seated; insufficient buffer; damaged electrodes. | Ensure lid is fully closed and magnetic sensor is engaged. Verify buffer level covers the gel and electrodes. Inspect and replace electrode cassettes if necessary. |
| Gel leaks from tank. | Improperly seated gel tray; damaged gel tank. | Ensure gel tray is correctly positioned in the tank. Inspect tank for cracks; replace if damaged. |
| pobrea sample migration or distorted bands. | Incorrect buffer concentration; air bubbles in wells; incorrect voltage/uneko ezarpenak. | Verify buffer preparation. Ensure no air bubbles are trapped during gel casting or sample loading. Check and adjust voltage/current settings according to protocol. |
8. Zehaztapen Teknikoak
Detailed specifications for the Labnet Enduro Gel XL Electrophoresis System (Model E0160-230V):
- Eredua: E0160-230V
- Potentzia sarrera: 230V
- Irteera boltage: 150V arte
- Irteera-korrontea: Gehienez 400mA
- Kontrola: Konstante Voltage or Constant Current with automatic crossover
- Gel Sizes Supported: 12.5 x 12cm, 12.5 x 6cm, 6 x 6cm
- Gehienezko Sample Edukiera: Gehienez 112 samples (with 12.5 x 12cm gel and multiple combs)
- Neurriak (paketea): 13.5 x 13.5 x 3 hazbete
- Pisua (Paketea): 3.45 kilo
- Fabrikatzailea: Labnet
- Jatorrizko herrialdea: AEB
9. Bermea eta Laguntza
For warranty information, technical support, or service inquiries regarding your Labnet Enduro Gel XL Electrophoresis System, please contact Labnet International directly. Refer to your purchase documentation for specific warranty terms and conditions.
Labnet Contact Information:
- Visit the official Labnet webwebgunea, harremanetarako datu eguneratuenak lortzeko.
- Refer to the contact information provided with your product packaging.
